Genotypes Variation of Interferon-gamma gene with chronic hypertension risk

Abstract

The issue of interferon-gamma (IFN-γ) T/A +874 gene polymorphism has been assessed in various ethnicities with hypertension, and inconsistent outcomes have been provided. Hence, this study is devoted to the genetics study for interferon-gamma gene polymorphism in position T/A +874 for hypertension patients (mean age 56.95±1.9), and the control group (mean age 41.3±2.1). To meet that aim,  ARMS-PCR (Amplification refractory mutation system technique) is utilized. The electrophoresis for IFN-γ +874 T/A gene revealed the presence of two alleles A and T, with three genotypes AA, TA, and TT. The results show a high frequency for allele T (67.11%) for IFN-γ +874 T/A gene as compared with A allele (32.88%) in hypertension patients’ samples and related with an etiological fraction (EF) of hypertension dependent on values of odds ratio (4.52) and confidence intervals 95% (3.09 to 6.61), while A allele associated with a preventive fraction (PF) of hypertension. The ARMS-PCR analysis results present a high frequency of TT (homozygote) and TA (heterozygote) genotypes of IFN-γ T/A +874 gene in hypertension patients (46.32% and 41.52%, respectively) as compared with control (16.67% and 28.89%, respectively) and associated with Etiological fraction in hypertension risk, and OR was 4.31 with CI 2.32 to 8.02 and the etiological fraction was 35.6% for TT genotype while TA was of OR 1,75 and CI 95% from 1.02 to 3.00. The AA homozygote genotype presents high frequency in control (54.44%) as compared with hypertension patients (12.11%) and is associated with Preventive fraction. the OR was 0.12 with a CI of 95% from 0.06 to 0.21.