Application of microwave against Protoscoleces of Echinococcus granulosus in vitro and Secondary Hydatid Disease in BALB/c Mice
Keywords:
Echinococcus granulosus, microwave`, Hydatidosis, BALB/c MiceAbstract
The present research examines the impact of microwave of (230-240V-50Hz 900W) on the viability of Echinococcus granulosus protoscoleces in vitro for different exposing periods, 3, 5, 7, 10, 12, 13, and 15 seconds, respectively. Subsequently, the study investigates the prevention of mice against infestation with secondary hydatid cysts, compared with the control animals infested with protoscoleces without exposure to microwaves over four months, depending on many criteria, including the numbers, weights, and diameters of developed hydatid cysts. The results revealed an obvious infl of microwaves on Echinococcus granulosus protoscoleces viability by an increase of exposure period in vitro, 90%, 80%, 77%, 70%, 50, 30, and 0%, respectively. Plus, the results showed a decrease of 97%, 92.5% of hydatid cysts numbers in the mice injected with exposed protoscoleces with 30% fertility exposed for 13 seconds, four months post-infection, followed by 92.5% and 87.2% in the same group, two and three months post-infection.
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