1) Investigación >
Artículos Publicados >

Por favor, use este identificador para citar o enlazar este ítem:

Título : Effect of aliphatic, monocarboxylic, dicarboxylic, heterocyclic and sulphur-containing amino acids on Leishmania spp. chemotaxis
Autor : Diaz, E.
Zacarias, A.K.
Pérez, S.
Vanegas, O.
Köhidai, L.
Padrón-Nieves, M.
Ponte-Sucre, Alicia
Palabras clave : Amino acids
flagellum length
Leishmania braziliensis
Leishmania amazonensis
parasite’s motility
osmotic stress
Fecha de publicación : 26-Dec-2016
Citación : Parasitology;142(13):1621-1630, 2015.
Resumen : In the sand-fly mid gut, Leishmania promastigotes are exposed to acute changes in nutrients, e.g. amino acids (AAs). These metabolites are the main energy sources for the parasite, crucial for its differentiation and motility. We analysed the migratory behaviour and morphological changes produced by aliphatic, monocarboxylic, dicarboxylic, heterocyclic and sulphur-containing AAs in Leishmania amazonensis and Leishmania braziliensis and demonstrated that L-methionine (10−12 M), L-tryptophan (10−11 M), L-glutamine and L-glutamic acid (10−6 M), induced positive chemotactic responses, while L-alanine (10−7 M), L-methionine (10−11 and 10−7 M), L-tryptophan (10−11 M), L-glutamine (10−12 M) and L-glutamic acid (10−9 M) induced negative chemotactic responses. L-proline and L-cysteine did not change the migratory potential of Leishmania. The flagellum length of L. braziliensis, but not of L. amazonensis, decreased when incubated in hyperosmotic conditions. However, chemo-repellent concentrations of L-alanine (Hypo-/hyper-osmotic conditions) and L-glutamic acid (hypo-osmotic conditions) decreased L. braziliensis flagellum length and L-methionine (10−11 M, hypo-/hyper-osmotic conditions) decreased L. amazonensis flagellum length. This chemotactic responsiveness suggests that Leishmania discriminate between slight concentration differences of small and structurally closely related molecules and indicates that besides their metabolic effects, AAs play key roles linked to sensory mechanisms that might determine the parasite’s behaviour.
ISSN : 0031-1820
Aparece en las colecciones: Artículos Publicados

Ficheros en este ítem:

Fichero Descripción Tamaño Formato
Diaz et al. 2015.pdf335.79 kBAdobe PDFVisualizar/Abrir

Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2008 MIT and Hewlett-Packard - Comentarios