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http://hdl.handle.net/10872/12326
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| Título : | Microwave irradiation for rapid epidermis-dermis separation and improved epidermal cell immunodetection |
| Autor : | Sánchez, MA
Díaz, NL
Tapia, Félix J. |
| Palabras clave : | Dentritic epidermal cells
Epidermal
Dermal separation
Immunohistology
Langerhans cells
Microwave |
| Fecha de publicación : | 26-Apr-2002 |
| Editorial : | BIOTECHNIC AND HISTOCHEMISTRY |
| Citación : | Vol. 77;4 |
| Resumen : | We explored the effects of microwave irradiation on epidermal-dermal separation and subsequent immunostaining of epidermal cells. Epidermal sheets were obtained after incubation in 0.02 M EDTA in PBS and microwave irradiation with 4 pulses of 420 watts for 5 sec, with a total incubation period of 4 min. The control epidermal sheets were immunostained for Langerhans cells and dendritic epidermal T cell using a conventional immunoperoxidase method. The experimental immunodetection of these cells was assisted by incubating the primary antibodies for 10 min at 70 watts. We showed a simple and rapid method for separation of the epidermal-dermal junction and immunostaining of epidermal cells with optimal morphological preservation. |
| URI : | http://hdl.handle.net/10872/12326 |
| Aparece en las colecciones: | Artículos Publicados
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